Bogdanova N P, Nikol'skiĭ N N, Semenova E G
Tsitologiia. 1982 Jun;24(6):667-72.
The transport of L-alanine in cultured 3T3, 3T6 and CHO cells has been studied. The estimated Michaelis kinetic constants KM and Vmax for 3T3, 3T6 and CHO cells were 0.6 mM and 3 mM/min, 2.5 mM and 8.3. mM/min, 0.7 mM and 8.4 mM/min, respectively. Alanine transport was inhibited by glycine, serine, alpha-aminoisobutyric acid, methionine, and in a lesser degree by leucine and valine. Glycine depressed alanine transport with the constant of inhibition (Ki) similar to the constant of glycine transport (Km). Alanine transport was strictly diminished in a Na+-dependent medium at the expense of the increase of Km for transport. It is concluded that in 3T3, 3T6 and CHO cells, alanine is transported by the Na+-dependent "A" system.
对培养的3T3、3T6和CHO细胞中L-丙氨酸的转运进行了研究。3T3、3T6和CHO细胞的米氏动力学常数KM和Vmax估计值分别为0.6 mM和3 mM/分钟、2.5 mM和8.3 mM/分钟、0.7 mM和8.4 mM/分钟。丙氨酸转运受到甘氨酸、丝氨酸、α-氨基异丁酸、蛋氨酸的抑制，亮氨酸和缬氨酸的抑制作用较小。甘氨酸抑制丙氨酸转运的常数（Ki）与甘氨酸转运常数（Km）相似。在依赖Na+的培养基中，丙氨酸转运严格减少，代价是转运的Km增加。得出结论，在3T3、3T6和CHO细胞中，丙氨酸通过依赖Na+的“A”系统进行转运。
