Lubec G, Latzka U, Coradello H, Pollak A
Wien Klin Wochenschr. 1982 May 28;94(11):288-90.
Though there is sufficient evidence that fibronectin is an integral extracellular matrix protein of the normal human kidney, the distribution and the interaction with structural proteins of the kidney have not been resolved. There are disagreements between investigators whether fibronectin is a component of the glomerular basement membrane or linked to it. We have been examining the interaction between the glomerular basement membrane and its components type IV collagen and laminin and fibronectin. Applying affinity chromatography we detected significant interaction between collagen type IV and laminin, and fibronectin on the other hand. The noncovalent interaction between collagen type IV and fibronectin was reversible after elution with 2 M urea, the association between laminin and fibronectin was even stronger and reversible applying 3 M urea for elution. The glomerular basement membrane-fibronectin interaction reflected the laminin pattern though minor binding sites at 1 M urea and 2 M urea could be detected. These interaction studies showed the chemical and thermodynamical possibilities and probability of the glomerular basement membrane and fibronectin linkage.
尽管有充分证据表明纤连蛋白是正常人类肾脏不可或缺的细胞外基质蛋白，但其在肾脏中的分布以及与肾脏结构蛋白的相互作用尚未明确。研究人员对于纤连蛋白是肾小球基底膜的组成成分还是与之相连存在分歧。我们一直在研究肾小球基底膜与其成分Ⅳ型胶原、层粘连蛋白和纤连蛋白之间的相互作用。通过亲和层析，我们检测到Ⅳ型胶原与层粘连蛋白之间存在显著相互作用，另一方面，也检测到了Ⅳ型胶原与纤连蛋白之间的相互作用。用2M尿素洗脱后，Ⅳ型胶原与纤连蛋白之间的非共价相互作用是可逆的，而层粘连蛋白与纤连蛋白之间的结合更强，用3M尿素洗脱时也是可逆的。肾小球基底膜 - 纤连蛋白的相互作用虽反映了层粘连蛋白的模式，但在1M尿素和2M尿素浓度下也能检测到少量结合位点。这些相互作用研究展示了肾小球基底膜与纤连蛋白连接的化学和热力学可能性及概率。
