Santos-Ruiz A, Cascales C, Martín-Sanz P, Cascales M
Acta Vitaminol Enzymol. 1982;4(1-2):69-80.
The administration of ethanol plus fat diet has been investigated in our laboratory in relation to the experimental fatty liver in rats. Considering that lipid metabolism are markedly altered in these experimental conditions, the present work studies the effect of simultaneous administration of Coenzyme A (CoA) on the alterations produced by ethanol and lipid diet on glycolytic and lipogenic routes. Ethanol was administered in a 15% solution as the only drinking fluid and a high fat diet (45% vegetable fat as total calories) for three months. CoA was intraperitoneally administered at a dose level of 1.0 mg/Kg body weight/day. Enzymatic activities were determined in the soluble fraction of liver homogenates. The methods used for the estimation of the enzymatic activities are described by Bergmeyer. The most significant changes found by the effect of CoA administration correspond to malic enzyme, citrate lyase and 6-phosphogluconate dehydrogenase. Fatty acid synthetase and glycolytic kinases, especially diminished by the effect of fat diet, did not show any significant restoration of their activities when ethanol and CoA were simultaneously administered.
在我们实验室中，已经对乙醇加高脂饮食与大鼠实验性脂肪肝的关系进行了研究。考虑到在这些实验条件下脂质代谢会发生显著改变，本研究探讨了同时给予辅酶A（CoA）对乙醇和高脂饮食所引起的糖酵解和脂肪生成途径改变的影响。乙醇以15%的溶液作为唯一的饮用水给予，同时给予高脂饮食（占总热量的45%的植物脂肪），持续三个月。CoA以1.0毫克/千克体重/天的剂量腹腔注射。在肝脏匀浆的可溶性部分测定酶活性。用于估算酶活性的方法由Bergmeyer描述。发现给予CoA所产生的最显著变化与苹果酸酶、柠檬酸裂解酶和6-磷酸葡萄糖酸脱氢酶有关。脂肪酸合成酶和糖酵解激酶，特别是受高脂饮食影响而降低的，在同时给予乙醇和CoA时，其活性并未显示出任何显著恢复。
