Pike I L, Wales R G
Aust J Biol Sci. 1982;35(2):195-206. doi: 10.1071/bi9820195.
Rate of [14C]glucose uptake by mouse embryos during in vitro culture in media containing optimal concentrations of lactate and pyruvate increased throughout preimplantation development. A reduction in the glucose concentration of the medium from 5.56 to 0.28 mM resulted in a two- to fivefold decrease in glucose metabolism, suggesting near-saturation of the membrane carrier at the lower concentration. Changes in the level of lactate and pyruvate in the medium had little effect on glucose metabolism after the third cleavage division. However, further evidence of interaction between energy substrates during the initial cleavage was obtained. Glucose was stored either as desmoglycogen during early cleavage or in a larger acid-soluble glycogen pool in the latter stages of development. The accumulation of glycogen calculated from its production by blastocysts cultured in either 5.56 or 0.28 mM glucose greatly exceeded that determined in blastocysts freshly collected from the uterus. The absence of lactate and pyruvate from the medium had only minimal effects on glycogen accumulation. Most of the glucose carbon was stored as a form of glycogen. However, considerable amounts were also found in acid-soluble material other than glycogen at all developmental stages, probably as the parent compound and its catabolites. Some was also present in lipids, nucleic acids and proteins.
在含有最佳浓度乳酸盐和丙酮酸盐的培养基中进行体外培养时，小鼠胚胎对[14C]葡萄糖的摄取率在植入前发育过程中持续增加。培养基中葡萄糖浓度从5.56 mM降至0.28 mM，导致葡萄糖代谢下降两到五倍，这表明在较低浓度下膜载体接近饱和。第三次卵裂分裂后，培养基中乳酸盐和丙酮酸盐水平的变化对葡萄糖代谢影响不大。然而，在最初的卵裂过程中获得了能量底物之间相互作用的进一步证据。葡萄糖在早期卵裂时以脱支糖原的形式储存，在发育后期则储存在更大的酸溶性糖原池中。根据在5.56 mM或0.28 mM葡萄糖中培养的囊胚产生的糖原计算，其积累量大大超过从子宫中新鲜收集的囊胚中测定的量。培养基中不含乳酸盐和丙酮酸盐对糖原积累的影响最小。大部分葡萄糖碳以糖原的形式储存。然而，在所有发育阶段，除糖原外的酸溶性物质中也发现了相当数量的葡萄糖，可能是作为母体化合物及其分解代谢产物。还有一些存在于脂质、核酸和蛋白质中。
