Rivest R S, Irwin D, Mandel H G
Biochem Pharmacol. 1982 Aug 1;31(15):2505-11. doi: 10.1016/0006-2952(82)90061-2.
The inhibition of protein synthesis by 8-azaguanine (azaG) in L1210 cells in culture was investigated. AzaG selectively inhibited protein synthesis at concentrations where viability was decreased significantly. AzaG altered the polyribosome sedimentation profile, increasing the numbers of monosomes and smaller polysomes and decreasing the number of larger polysomes. The reversal by cycloheximide of the alterations in the polysome profile suggested that azaG inhibited the initiation of translation. This was confirmed by the demonstration of inhibition of the formation of the 43S and 80S initiation complexes.
研究了培养的L1210细胞中8-氮杂鸟嘌呤（azaG）对蛋白质合成的抑制作用。在显著降低细胞活力的浓度下，azaG选择性地抑制蛋白质合成。azaG改变了多核糖体沉降图谱，增加了单体和较小多核糖体的数量，减少了较大多核糖体的数量。环己酰亚胺对多核糖体图谱改变的逆转表明azaG抑制翻译起始。对43S和80S起始复合物形成的抑制证明了这一点。
