气相色谱-质谱法鉴定和测定6-羟基-1-甲基-1,2,3,4-四氢-β-咔啉

Identification and measurement of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline by gas chromatography-mass spectrometry.

作者信息

Beck O, Bosin T R, Lundman A, Borg S

出版信息

Biochem Pharmacol. 1982 Aug 1;31(15):2517-21. doi: 10.1016/0006-2952(82)90063-6.

Abstract

A glass capillary gas chromatographic-mass spectrometric method for the analysis of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline in urine, plasma and blood platelets was developed. The method involves the use of a deuterated analogue as internal standard, addition of semicarbazide, extraction with methylene chloride and formation of pentafluoropropionyl derivatives. The 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline was found to occur in the urine of controls and alcoholics as both the free and conjugated compound. In the controls the mean level of free and conjugated 60MTHBC was 75 +/- 20 nmole/l. When intoxicated, the alcoholics had a statistically higher excretion rate of the conjugate(s) than the controls. The compound was not detectable in blood platelets or plasma.

摘要

建立了一种玻璃毛细管气相色谱 - 质谱法，用于分析尿液、血浆和血小板中的6 - 羟基 - 1 - 甲基 - 1,2,3,4 - 四氢 - β - 咔啉。该方法包括使用氘代类似物作为内标，加入氨基脲，用二氯甲烷萃取并形成五氟丙酰基衍生物。发现对照组和酗酒者尿液中的6 - 羟基 - 1 - 甲基 - 1,2,3,4 - 四氢 - β - 咔啉以游离和结合化合物的形式存在。对照组中游离和结合的60MTHBC平均水平为75±20纳摩尔/升。酗酒者在醉酒时，其结合物的排泄率在统计学上高于对照组。在血小板或血浆中未检测到该化合物。