Horie S, Ishii H, Orii H, Suga T
Biochim Biophys Acta. 1982 Aug 25;690(1):74-80. doi: 10.1016/0005-2736(82)90240-1.
Changes in membrane surface properties of hepatic peroxisomes of rats under several conditions were observed by aqueous polymer two-phase systems, which contained 6% (w/w) dextran T 500, 6% (w/w) polyethyleneglycol 4000, 250 mmol sucrose/kg and various concentrations of sodium phosphate buffer. The partition of peroxisomes into the upper phase depended to a large extent on their membrane surface charge. The cross-points of peroxisomes shifted from 5.55 to 5.25 and 5.2 after the administration of clofibrate and aspirin for 2 weeks, respectively, although that of alloxan-diabetic rat peroxisomes was not altered. The hydrophobic properties of peroxisomes, examined by means of a partition containing polyethyleneglycol monostearate, were altered by diabetes and starvation, but no change occurred in rats treated with clofibrate or aspirin. In the liver of rats fed a high-fat diet, the partition of peroxisomes was the same as that of the control. These findings indicate that hypolipidemic drugs such as clofibrate and aspirin induce the proliferation of peroxisomes and lead to the alteration of the surface charge of peroxisomal membranes. Diabetes or fasting lead to an alteration mainly of the hydrophobic properties. Both changes are probably due to alteration of content and/or composition of the proteins and the phospholipids in peroxisomal membrane under the conditions used.
采用含有6%（w/w）葡聚糖T 500、6%（w/w）聚乙二醇4000、250 mmol蔗糖/kg以及不同浓度磷酸钠缓冲液的水相聚合物双相系统，观察了几种条件下大鼠肝脏过氧化物酶体膜表面性质的变化。过氧化物酶体在上相中的分配在很大程度上取决于其膜表面电荷。分别给予氯贝丁酯和阿司匹林2周后，过氧化物酶体的交叉点分别从5.55移至5.25和5.2，而四氧嘧啶糖尿病大鼠过氧化物酶体的交叉点未改变。通过含有聚乙二醇单硬脂酸酯的分配来检测，过氧化物酶体的疏水性质因糖尿病和饥饿而改变，但用氯贝丁酯或阿司匹林治疗的大鼠未发生变化。在喂食高脂饮食的大鼠肝脏中，过氧化物酶体的分配与对照组相同。这些发现表明，氯贝丁酯和阿司匹林等降血脂药物可诱导过氧化物酶体增殖，并导致过氧化物酶体膜表面电荷的改变。糖尿病或禁食主要导致疏水性质的改变。这两种变化可能是由于在所使用的条件下，过氧化物酶体膜中蛋白质和磷脂的含量和/或组成发生了改变。
