Emmons G T, Rosenblum E R, Peace J N, Malloy J M, Doerfler D L, McManus I R, Campbell I M
Biomed Mass Spectrom. 1982 Jul;9(7):278-85. doi: 10.1002/bms.1200090703.
Radiogas chromatography, used in conjunction with mass spectrometry, has been used to analyze the sterol content of cultured chick muscle cells. Seven sterols, plus lanosterol, were detected. These sterols conformed to a linear biosynthetic pathway linking lanosterol and cholesterol. The reaction sequence is: C-14 demethylation, C-4 demethylation, delta 8 leads to delta 5 double bond rearrangement, delta 24 double bond reduction. When chick cells were treated with increasing concentrations of 20,25-diazacholesterol, components of this pathway and aberrant products accumulated. These accumulations suggest that diazacholesterol affects reductases, double bond isomerases and the C-14 demethylation enzymes of sterol biosynthesis.
放射气相色谱法与质谱联用，已被用于分析培养的鸡肌肉细胞中的甾醇含量。检测到七种甾醇，外加羊毛甾醇。这些甾醇符合将羊毛甾醇与胆固醇联系起来的线性生物合成途径。反应顺序为：C-14去甲基化、C-4去甲基化、δ8向δ5双键重排、δ24双键还原。当鸡细胞用浓度不断增加的20,25-二氮杂胆固醇处理时，该途径的成分和异常产物会积累。这些积累表明二氮杂胆固醇会影响甾醇生物合成中的还原酶、双键异构酶和C-14去甲基化酶。
