Alemán V, Trejo R, Morales E, Hernández-Jáuregui P, Delhumeau-Ongay G
J Reprod Fertil. 1978 Sep;54(1):67-75. doi: 10.1530/jrf.0.0540067.
A technique was developed to obtain enriched populations of large numbers of primary spermatacytes (70--81%) and of spermatids (75%) from immature rat testes (21--23 and 38 days old respectively) in a simple and rapid fashion. The cells were nearly all viable and membrane preservation was good. The testicular cells were dispersed by a mild mechanical treatment combined with an incubation with purified collagenase, and the cell populations were separated by centrifugation in a discontinuous dextran gradient in a cell culture medium.
开发了一种技术，可从未成熟大鼠睾丸（分别为21 - 23天和38天大）中以简单快速的方式获得大量富集的初级精母细胞（70 - 81%）和精子细胞（75%）群体。细胞几乎全部存活，细胞膜保存良好。通过温和的机械处理结合与纯化胶原酶孵育来分散睾丸细胞，并在细胞培养基中的不连续葡聚糖梯度中通过离心分离细胞群体。
