Gossett L A, Hecht R M, Epstein H F
Cell. 1982 Aug;30(1):193-204. doi: 10.1016/0092-8674(82)90025-3.
The differentiation of body-wall muscle cells was studied in the nematode Caenorhabditis elegans. Specific antibodies to myosin and paramyosin, major protein constituents of differentiated muscle, react with mesodermal cells in wild-type embryos towards the end of the first half of embryogenesis. Immunoreactive cells (2-16) first appear in embryos with 400-450 of the 550 cells present at hatching. Such embryos have developed at 25.5 degrees C for 4-4 1/2 hr beyond the two-cell stage. As development proceeds, a maximum of 81 immunoreactive cells forms four columns running anterior-posterior. Each column is composed of two lines of tightly opposed round cells, which then elongate into spindle-shaped cells. Mutant embryos in which cleavage arrests prematurely also generate cells that produce myosin and paramyosin. The initiation of muscle differentiation appears to be independent of the number of cell or nuclear divisions within a lineage or of the proliferation of other cells. These results suggest that the biosynthesis of muscle-specific proteins by nematode embryonic muscle cells is regulated by mechanisms intrinsic to these cells.
对线虫秀丽隐杆线虫体壁肌肉细胞的分化进行了研究。针对肌球蛋白和副肌球蛋白（分化肌肉的主要蛋白质成分）的特异性抗体，在胚胎发育前半期接近尾声时，与野生型胚胎中的中胚层细胞发生反应。免疫反应性细胞（2 - 16个）首先出现在孵化时550个细胞中已有400 - 450个细胞的胚胎中。此类胚胎在25.5摄氏度下，从二细胞阶段开始发育4 - 4.5小时。随着发育的进行，最多81个免疫反应性细胞形成四列，从前向后排列。每列由两行紧密相对的圆形细胞组成，这些细胞随后伸长成为纺锤形细胞。卵裂过早停止的突变胚胎也会产生产生肌球蛋白和副肌球蛋白的细胞。肌肉分化的起始似乎与一个谱系内的细胞或核分裂数量或其他细胞的增殖无关。这些结果表明，线虫胚胎肌肉细胞中肌肉特异性蛋白质的生物合成受这些细胞内在机制的调节。
