肌酸激酶转换因子测定法。

An assay for creatine kinase conversion factor.

作者信息

Falter H, Michelutti L, Mazzuchin A, Whiston W

出版信息

Clin Biochem. 1982 Aug;15(4):200-5. doi: 10.1016/s0009-9120(82)90096-0.

Abstract

The M subunits of creatine kinase (CK) isoenzymes, MM and MB, are subject to a postsynthetic modification in serum. As a result of this modification three sub-bands of MM can be identified by agarose electrophoresis, called MM3, MM2 and MM1 in the order of increasing electrophoretic mobility towards the anode. An assay has been developed for the protein, called creatine kinase conversion factor, which catalyses the reaction leading to the sub-bands. The assay is based on the rate of conversion of MM3 to MM2 and MM1. The first order rate constant for the conversion, designated as kc, for sera of 24 apparently healthy adults ranges from 0.0221 to 0.0740 hr-1 with a mean of 0.0392 hr-1 under the conditions of the assay. Temperature and pH dependence of kc have been determined.

摘要

肌酸激酶（CK）同工酶的M亚基，即MM和MB，在血清中会发生合成后修饰。由于这种修饰，通过琼脂糖电泳可识别出MM的三个亚条带，按照向阳极电泳迁移率增加的顺序分别称为MM3、MM2和MM1。已开发出一种针对一种名为肌酸激酶转换因子的蛋白质的检测方法，该因子催化导致这些亚条带形成的反应。该检测基于MM3向MM2和MM1的转换速率。在检测条件下，24名明显健康成年人血清的转换一级速率常数（指定为kc）范围为0.0221至0.0740 hr⁻¹，平均值为0.0392 hr⁻¹。已确定kc对温度和pH的依赖性。