Tasset J J, Hassan F M
Clin Chem. 1982 Oct;28(10):2154-7.
We describe a liquid-chromatographic procedure for amoxapine and 8-hydroxyamoxapine, its active metabolite, in serum. We used a mu-Bondapak C18 reversed-phase column and a mobile phase of acetonitrile/water (74/26 by vol) plus 26 microL of n-butylamine per liter. The compounds were measured at 254 nm, with 8-methoxyloxapine as internal standard. Necessary pre-analysis purification consisted of adsorbing the drug from serum onto extraction columns, eluting with 1-butanol/hexane (1/5 by vol), re-extracting into aqueous acid, and from that re-extracting again into the elution-solvent mixture. We prefer this procedure for monitoring both therapeutic and toxic concentrations of amoxapine, because parent drug and metabolite are measured separately.
我们描述了一种用于测定血清中阿莫沙平及其活性代谢物8-羟基阿莫沙平的液相色谱法。我们使用μ-Bondapak C18反相柱和乙腈/水（体积比74/26）加每升26微升正丁胺的流动相。化合物在254nm处测定，以8-甲氧基洛沙平作为内标。必要的分析前净化包括将血清中的药物吸附到萃取柱上，用1-丁醇/己烷（体积比1/5）洗脱，再萃取到酸性水溶液中，然后再次萃取到洗脱溶剂混合物中。我们更喜欢这个方法来监测阿莫沙平的治疗浓度和中毒浓度，因为母体药物和代谢物是分别测定的。
