一种用于测量红细胞转酮醇酶活性的连续流动（自动分析仪II）方法。

A continuous-flow (AutoAnalyzer II) procedure for measuring erythrocyte transketolase activity.

作者信息

Waring P P, Fisher D, McDonnell J, McGown E L, Sauberlich H E

出版信息

Clin Chem. 1982 Nov;28(11):2206-13.

Abstract

In this semiautomated method, an AutoAnalyzer II is used to measure the enzymic production of glyceraldehyde 3-phosphate in hemolysates, to assay erythrocyte transketolase (EC 2.2.1.1) activity. Hemolysate and indicator reactions are separated by dialysis to eliminate hemoglobin interference and increase sensitivity. Internal standards of glyceraldehyde 3-phosphate in hemolysate carriers were quantitatively measured with good precision and accuracy in the presence or absence of the transketolase substrate, ribose 5-phosphate. Chart-recorder values for these standards were used to calibrate the AutoAnalyzer output in IUB units (U) of transketolase activity. Substrate-product relationships were examined to characterize reaction kinetics and optimize assay conditions. AutoAnalyzer transketolase results correlated well with those from two manual procedures.

摘要

在这种半自动方法中，使用自动分析仪II来测量溶血产物中3-磷酸甘油醛的酶促生成，以测定红细胞转酮醇酶（EC 2.2.1.1）活性。溶血产物反应和指示剂反应通过透析分离，以消除血红蛋白的干扰并提高灵敏度。在存在或不存在转酮醇酶底物5-磷酸核糖的情况下，对溶血产物载体中3-磷酸甘油醛的内标进行了精密度和准确度良好的定量测量。这些标准品的图表记录仪值用于将自动分析仪的输出校准为转酮醇酶活性的国际单位（U）。研究了底物-产物关系以表征反应动力学并优化测定条件。自动分析仪的转酮醇酶结果与两种手动方法的结果相关性良好。