12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯对青年和老年成年人外周血淋巴细胞的促有丝分裂活性。

Mitogenic activity of 12-O-tetradecanoyl phorbol-13-acetate on peripheral blood lymphocytes from young and aged adults.

作者信息

Nagel J E, Chrest F J, Adler W H

出版信息

Clin Exp Immunol. 1982 Jul;49(1):217-24.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1536658/

Abstract

The effect of age on the proliferative response to 12-O-tetradecanoyl phorbol-13-acetate (TPA) was examined using peripheral blood lymphocytes from 185 adults. TPA-induced DNA synthesis measured by cellular 3H-thymidine incorporation was found, like the responses of cells activated by PHA and Con A, to markedly diminish with advancing age. The presence of indomethacin (1 microgram/ml) or Ro 20-5720 (10 micrograms/ml) in TPA activated cell cultures, unlike PHA stimulated cultures, did not result in augmentation of 3H-thymidine incorporation by cells from elderly individuals. These results demonstrate that prostaglandin synthesizing suppressor cells are not responsible for the age-related depression of cellular immune function observed in TPA activated cells and confirm the observation that decreased production and/or utilization of soluble mediators, such as IL-2, may account for the diminished mitogen responsiveness of lymphocytes from elderly individuals.

摘要

利用185名成年人的外周血淋巴细胞，研究了年龄对12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）增殖反应的影响。通过细胞3H - 胸腺嘧啶核苷掺入法测定的TPA诱导的DNA合成，与PHA和Con A激活的细胞反应一样，随着年龄的增长而显著减少。在TPA激活的细胞培养物中加入消炎痛（1微克/毫升）或Ro 20 - 5720（10微克/毫升），与PHA刺激的培养物不同，并未导致老年个体细胞的3H - 胸腺嘧啶核苷掺入增加。这些结果表明，前列腺素合成抑制细胞与TPA激活细胞中观察到的与年龄相关的细胞免疫功能下降无关，并证实了以下观察结果：可溶性介质如IL - 2的产生和/或利用减少可能是老年个体淋巴细胞丝裂原反应性降低的原因。

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本文引用的文献

1

Binding of [3H]12-O-tetradecanoylphorbol-13-acetate to intact human peripheral blood lymphocytes.[3H]12-十四酰佛波醇-13-乙酸酯与完整人类外周血淋巴细胞的结合

Cancer Res. 1980 Apr;40(4):1119-24.

2

Failure of lymphokine-producing lymphocytes from aged humans to undergo activation by recall antigens.老年人产生淋巴因子的淋巴细胞无法被回忆抗原激活。

J Immunol. 1980 May;124(5):2169-74.

3

Relationship of cell cycle to recovery of IL 2 activity from human mononuclear cells, human and mouse T cell lines.细胞周期与人类单核细胞、人类和小鼠T细胞系中白细胞介素2活性恢复的关系。

J Immunol. 1981 Nov;127(5):1936-40.

4

Immunological studies of aging. Decreased production of and response to T cell growth factor by lymphocytes from aged humans.衰老的免疫学研究。老年人淋巴细胞产生T细胞生长因子的能力及其对T细胞生长因子反应性的降低。

J Clin Invest. 1981 Apr;67(4):937-42. doi: 10.1172/jci110143.

5

The tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces the production of lymphocyte-activating factors (LAP) in human lymphocyte cultures.肿瘤促进剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）可在人淋巴细胞培养物中诱导淋巴细胞激活因子（LAP）的产生。

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6

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