牛磺酸对大鼠[D-丙氨酸2，甲硫氨酸5]脑啡肽酰胺耐受性的影响。

Effects of taurine on tolerance to [D-Ala2, Met5]enkephalinamide in rats.

作者信息

Izumi K, Munekata E, Barbeau A, Nakanishi T, Yoshida M, Yamamoto H, Fukuda T

出版信息

Eur J Pharmacol. 1982 Aug 13;82(1-2):55-63. doi: 10.1016/0014-2999(82)90552-0.

Abstract

Effects of taurine on tolerance to [D-Ala2, Met5]enkephalinamide (DAME) were investigated in rats. Tolerance was produced by five intraventricular administrations of DAME (50 microgram) during 3 consecutive days. The magnitude of developed tolerance to DAME was not uniform for each behavioral parameter; tolerance to analgesia effects developed more intensively and rapidly from the repeated injections of the peptide than that to akinesia effects. Pretreatment with taurine (9.5 X 10(-2) M) which was injected in a volume of 10 microliter intraventricularly 10 min prior to every administration of DAME suppressed the development of tolerance to both analgesia and akinesia effects of this peptide, whereas pretreatment with L-leucine at the same concentration did not. Spontaneous locomotor activity was measured for 1 h after the 90-min behavioral observation period was completed. That activity increased with the number of the peptide injections. Taurine pretreatment inhibited the induction of 'hyper'-locomotor activity. These results support the view that taurine may possess an ability to inhibit development of tolerance to morphine-like peptides in rats.

摘要

研究了牛磺酸对大鼠对[D-丙氨酸2，甲硫氨酸5]脑啡肽酰胺（DAME）耐受性的影响。连续3天通过脑室内注射5次DAME（50微克）产生耐受性。对于每个行为参数，对DAME产生的耐受性程度并不一致；与对运动不能效应的耐受性相比，对肽重复注射产生的镇痛效应的耐受性发展得更强烈、更快。在每次注射DAME前10分钟，以10微升的体积脑室内注射牛磺酸（9.5×10⁻² M）进行预处理，可抑制对该肽镇痛和运动不能效应的耐受性发展，而以相同浓度的L-亮氨酸进行预处理则无此作用。在90分钟行为观察期结束后，测量1小时的自发运动活动。该活动随肽注射次数增加。牛磺酸预处理抑制了“过度”运动活动的诱导。这些结果支持这样的观点，即牛磺酸可能具有抑制大鼠对吗啡样肽耐受性发展的能力。