Kramer I M, Hopkins C R
Mol Cell Endocrinol. 1982 Oct;28(2):191-8. doi: 10.1016/0303-7207(82)90031-4.
The regulation of prolactin (PRL) release by dopamine has been studied using short-term dissociated rat pituitary cell cultures. Concentrations of dopamine between 10(-12) and 10(-10) M stimulate PRL release, whereas concentrations between 10(-9) and 10(-7) M inhibit. By studying the kinetics of PRL release it was found that (1) the rate of PRL release increases only after a post-stimulus delay of 20 min, and (2) the response is phasic, stimulatory intervals being separated by intervals in which the rate of PRL release falls below the level of basal release. Inhibition of PRL release by dopamine occurs within 10 min after adding dopamine and is maintained tonically for a least 2 h. Cells treated with non-specific secretogogue (57 mM K+) during the inhibitory phases of dopamine-regulated release, are stimulated to secrete significant amounts of hormone.
利用短期解离的大鼠垂体细胞培养物研究了多巴胺对催乳素（PRL）释放的调节作用。多巴胺浓度在10^(-12)至10^(-10) M之间刺激PRL释放，而浓度在10^(-9)至10^(-7) M之间则起抑制作用。通过研究PRL释放的动力学发现：（1）PRL释放速率仅在刺激后延迟20分钟后才增加；（2）反应是阶段性的，刺激间隔被PRL释放速率低于基础释放水平的间隔分隔开。多巴胺对PRL释放的抑制作用在加入多巴胺后10分钟内发生，并持续紧张性抑制至少2小时。在多巴胺调节释放的抑制阶段用非特异性促分泌剂（57 mM K+）处理细胞，可刺激细胞分泌大量激素。
