David A, Levinsky H, Malik Z, Langzam Y, Feller N, Shaked P, Allalouf D
Gynecol Obstet Invest. 1982;14(3):195-201. doi: 10.1159/000299449.
Oviduct fluid was collected from 24 mature female rabbits in estrus fitted with a copper intrauterine device (IUD) in one of the uterine horns by laparotomy and examined for total sugar and protein content. The percentage of total sugar in the dry protein-containing material from the experimental side was 7.3 compared to 5.2 from the control side. The percentage of total protein was 67.0% of the total dry material from the experimental side, compared to 77.7% in the control side. Cellulose acetate electrophoresis confirmed these findings, demonstrating an increase in the strength of the periodic acid-Schiff stain bands of the glycoproteins from the copper IUD side and a weaker Ponceau-S stain of the protein bands. The ampullary-isthmic area and the uterotubal junction of both oviducts were examined with the scanning electron microscope. The secretory and ciliary cells on the copper IUD side were damaged in these two areas. These changes can be attributed to the presence and action of the copper ions in the oviduct milieu.
通过剖腹术从24只处于发情期的成熟雌性兔子身上收集输卵管液，这些兔子的一个子宫角内放置了铜宫内节育器（IUD），并检测其总糖和蛋白质含量。实验侧含蛋白质干物质中的总糖百分比为7.3%，而对照侧为5.2%。实验侧总蛋白质占总干物质的百分比为67.0%，而对照侧为77.7%。醋酸纤维素电泳证实了这些结果，显示铜宫内节育器一侧糖蛋白的过碘酸希夫染色带强度增加，蛋白质带的丽春红S染色较弱。用扫描电子显微镜检查双侧输卵管的壶腹-峡部区域和子宫输卵管交界处。铜宫内节育器一侧的这两个区域的分泌细胞和纤毛细胞受损。这些变化可归因于输卵管环境中铜离子的存在和作用。
