Ema M, Kanoh S
Nihon Yakurigaku Zasshi. 1982 May;79(5):369-81.
We performed studies on the mechanisms for the teratogenicity of trypan blue (TB) that is known as a potent teratogenic dye in rodents. TB was administered to Wistar rats at various doses and at different stages of gestation. Teratogenicity and embryolethality were observed by a single (50 and 250 mg/kg) subcutaneous injection and three (25 and 50 mg/kg) subcutaneous injections of TB daily from day 7 of pregnancy. The incidences of malformations in groups given 50 and 250 mg/kg on day 7 were 12 and 59%, respectively. Most of the fetuses with external malformations were accompanied with skeletal and/or internal anomalies. The types of frequently occurring malformations were as follows: exencephaly, spina bifida, tail anomaly, vertebral deformity, hydrocephaly and heart anomaly. Fetal toxicity was decreased after treatment with the mixture of TB and normal rat serum. The serum level of TB in pregnant rats increased to 308 micrograms/ml at one hour after subcutaneous treatment with TB 50 mg/kg and decreased rapidly, but remained at 58 micrograms/ml 72 hours later. Lower serum levels of TB were observed in pregnant rats given TB with serum. No fetotoxic effects of serum from pregnant rats treated with TB were observed in recipient rats given the serum on day 7, 8 and 9 of pregnancy.
我们对锥虫蓝（TB）的致畸机制进行了研究，锥虫蓝在啮齿动物中是一种强效致畸染料。在不同孕期阶段，以不同剂量给Wistar大鼠注射TB。从妊娠第7天开始，通过单次皮下注射（50和250mg/kg）以及每日三次皮下注射（25和50mg/kg）TB来观察致畸性和胚胎致死率。在第7天给予50和250mg/kg剂量组中，畸形发生率分别为12%和59%。大多数有外部畸形的胎儿伴有骨骼和/或内部异常。常见的畸形类型如下：无脑儿、脊柱裂、尾部异常、脊柱畸形、脑积水和心脏异常。用TB与正常大鼠血清的混合物处理后，胎儿毒性降低。皮下注射50mg/kg TB后1小时，孕鼠血清中TB水平升至308微克/毫升，并迅速下降，但72小时后仍维持在58微克/毫升。给孕鼠注射TB并同时注射血清时，观察到血清中TB水平较低。在妊娠第7、8和9天给接受血清的大鼠注射经TB处理的孕鼠血清，未观察到胎儿毒性作用。
