Maslansky C J, Williams G M
In Vitro. 1982 Aug;18(8):683-93. doi: 10.1007/BF02796423.
Hepatocyte primary cultures (HPC) derived from rat, mouse, hamster, and rabbit liver were characterized for a variety of parameters. The conditions that maximized recovery, attachment, and survival varied between species. Hepatocytes from all four species were capable of attaching in serum-free Williams' medium E (WME), but optimal attachment as monolayer cultures was achieved for mouse and hamster HPC in medium receiving 1% calf serum supplementation. Hamster hepatocytes required additional cations, whereas rabbit and rat hepatocytes displayed maximal attachment in medium supplemented with 10% calf serum. Survival of mouse and rabbit hepatocytes after 24 h in serum supplemented media was in the order of 90%. Rat and hamster hepatocyte 24 h survival was approximately 70 and 60%, respectively, and was not significantly affected by serum supplementation. Hepatocytes from each species varied in their content of cytochrome P450 at the time of isolation and in the rate of reduction during culture. Mouse and rat hepatocytes demonstrated the most rapid decline in content during the initial 24 h in culture, whereas concentrations in rabbit hepatocytes were virtually unchanged. The rate of decline in P450 concentrations in hamster hepatocytes was intermediate between those displayed by rat and rabbit hepatocytes. These studies have delineated conditions useful for the culture of hepatocytes from four species and have documented the status of an important parameter of their functional capability.
对源自大鼠、小鼠、仓鼠和兔肝脏的原代肝细胞培养物（HPC）进行了多种参数的表征。使回收率、贴壁率和存活率最大化的条件因物种而异。所有这四个物种的肝细胞都能够在无血清的威廉姆斯E培养基（WME）中贴壁，但对于小鼠和仓鼠的HPC，在添加1%小牛血清的培养基中作为单层培养物可实现最佳贴壁。仓鼠肝细胞需要额外的阳离子，而兔和大鼠的肝细胞在添加10%小牛血清的培养基中显示出最大贴壁率。在添加血清的培养基中培养24小时后，小鼠和兔肝细胞的存活率约为90%。大鼠和仓鼠肝细胞24小时的存活率分别约为70%和60%，且不受血清添加的显著影响。每个物种的肝细胞在分离时细胞色素P450的含量以及培养期间的降低速率各不相同。小鼠和大鼠肝细胞在培养的最初24小时内含量下降最快，而兔肝细胞中的浓度几乎没有变化。仓鼠肝细胞中P450浓度的下降速率介于大鼠和兔肝细胞之间。这些研究已经描绘了对四种物种的肝细胞进行培养有用的条件，并记录了其功能能力的一个重要参数的状况。
