Lee R E, Davison P F, Cintron C
Invest Ophthalmol Vis Sci. 1982 Nov;23(5):660-5.
Linear nonperforating incisions were made in the corneas of 2-week-old and 2-year-old rabbits. The resulting wounds were examined by light microscopy and transmission and scanning electron microscopy. A corneal incision of a 2-week-old rabbit produced a wide gaping wound caused by retraction of the cut stromal lamellae away from the incision. The wound became wider with time as the developing eye enlarged and the cut lamellae retracted further. Polymorphonuclear leukocytes, presumably from the tear film, penetrating into the wound area before it was covered over by the sliding epithelium. Most of the leukocytes disappeared by 3 days after wounding. Three to six layers of fibroblasts appeared beneath the epithelial plug. The tissue eventually rebuilt approximately one third of the corneal depth lost to the wound. The stroma of the wounded region did not return to its normal width, but the epithelium was thicker than that of the unwounded cornea. An incision in a 2-year-old rabbit cornea produced a narrow V-shaped wound that did not change shape with time. This wound was repaired by fibroblasts resulting in collagenous repair tissue being the same depth as the normal stroma. There appears to be no evidence for wide gaping wounds in humans in the literature, as was found in this study in rabbits.
在2周龄和2岁兔子的角膜上制作线性非穿孔切口。通过光学显微镜、透射电子显微镜和扫描电子显微镜对产生的伤口进行检查。2周龄兔子的角膜切口产生了一个宽大的裂开伤口，这是由于切割的基质板层从切口处回缩所致。随着发育中的眼睛增大以及切割的板层进一步回缩，伤口随着时间变得更宽。多形核白细胞大概来自泪膜，在伤口被滑动上皮覆盖之前穿透到伤口区域。大多数白细胞在受伤后3天消失。上皮栓下方出现三到六层成纤维细胞。组织最终重建了因伤口而损失的大约三分之一角膜深度。受伤区域的基质没有恢复到正常宽度，但上皮比未受伤的角膜更厚。2岁兔子角膜的切口产生了一个狭窄的V形伤口，其形状不会随时间改变。这个伤口由成纤维细胞修复，导致胶原修复组织与正常基质深度相同。正如本研究在兔子中发现的那样，文献中似乎没有证据表明人类存在宽大的裂开伤口。
