Z带：85,000道尔顿的无定形肌动蛋白和α-辅肌动蛋白及其与结构的关系。
The Z-band: 85,000-dalton amorphin and alpha-actinin and their relation to structure.
作者信息
Chowrashi P K, Pepe F A
出版信息
J Cell Biol. 1982 Sep;94(3):565-73. doi: 10.1083/jcb.94.3.565.
Abstract
The conclusions arrived at as a result of this work can be summarized as follows: (a) We have found that there is an 85,000-dalton protein, which we have called 85K amorphin, associated with the Z-band of chicken pectoralis muscle myofibrils. We have isolated and purified this protein. It is not a structural component of the Z-filaments since it can be extracted completely without extraction of the Z-filaments. Extraction of 85K amorphin results in loss of specific staining of the Z-band with fluorescence specific anti-85K amorphin. (b) We have found that alpha-actinin is the structural component of the Z-filaments, since extraction of alpha-actinin is accompanied by loss of the Z-filament structure.
摘要
这项工作得出的结论可总结如下
(a) 我们发现有一种85000道尔顿的蛋白质，我们将其称为85K无定形蛋白，它与鸡胸肌肌原纤维的Z带相关。我们已经分离并纯化了这种蛋白质。它不是Z细丝的结构成分，因为它可以在不提取Z细丝的情况下被完全提取出来。提取85K无定形蛋白会导致用荧光特异性抗85K无定形蛋白对Z带进行特异性染色时染色消失。(b) 我们发现α-辅肌动蛋白是Z细丝的结构成分，因为提取α-辅肌动蛋白会伴随着Z细丝结构的消失。
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