使用荧光试剂4-溴甲基-7-乙酰氧基香豆素的前列腺素灵敏高效液相色谱法

Sensitive high-performance liquid chromatographic method for prostaglandins using a fluorescence reagent, 4-bromomethyl-7-acetoxycoumarin.

作者信息

Tsuchiya H, Hayashi T, Naruse H, Takagi N

出版信息

J Chromatogr. 1982 Sep 10;231(2):247-54. doi: 10.1016/s0378-4347(00)81849-6.

Abstract

High-performance liquid chromatography of prostaglandins is developed in which a fluorescence reagent, 4-bromomethyl-7-acetoxycoumarin is used to perform the high-sensitivity detection. The reagent reacts with prostaglandins and related compounds to form the ester derivatives, which are separated using a reversed-phase system. Each labeled compound eluted from the column is successively hydrolyzed to the fluorescent coumarin derivative, and this fluorophore is introduced into a flow-through fluorometer. Prostaglandins can be determined in the range of at least 1 nmol to 5 pmol, and the detection limit is about 10 fmol. This system is applied to the analysis of prostaglandins in human seminal fluid.

摘要

开发了前列腺素的高效液相色谱法，其中使用荧光试剂4-溴甲基-7-乙酰氧基香豆素进行高灵敏度检测。该试剂与前列腺素及相关化合物反应形成酯衍生物，使用反相系统进行分离。从柱上洗脱的每种标记化合物依次水解为荧光香豆素衍生物，然后将该荧光团引入流通式荧光计中。前列腺素的测定范围至少为1 nmol至5 pmol，检测限约为10 fmol。该系统应用于人类精液中前列腺素的分析。