Lee J C, Gauntt C
J Interferon Res. 1982;2(3):345-54. doi: 10.1089/jir.1982.2.345.
Incubation of HeLa cells with human alpha interferon (HuIFN-alpha) resulted in increased phosphorylation of total ribosomal proteins (ribosome-associated and ribosome structural proteins) above that found for cells incubated in medium alone. Maximum phosphorylation of these proteins occurred with 4000 units/ml of HuIFN-alpha, 1-2 h of incubation of cells with HuIFN-alpha and a 32P pulse period of 1 h. Fractionation of total ribosomal proteins into ribosome-associated and 80S ribosomal structural proteins showed that the interferon-induced increase in phosphorylation was associated only with a 36K ribosome-associated polypeptide and phosphorylation of 80S ribosomal structural proteins was inhibited in interferon-treated cells. The level of inhibition of phosphorylation of ribosomal structural proteins in large and small subunits in interferon-treated cells was 14-19% and 76-81%, respectively. The inhibition of phosphorylation of ribosomal structural proteins persisted for 24 h following an initial 2 h of incubation of cells with interferon. Interferon treatment inhibited phosphorylation of several proteins associated with purified 80S ribosomes. Interferon treatment considerably reduced yields of coxsackievirus B3 in HeLa cells, but had little to no effect on rates of protein synthesis during 10 h of incubation of cells with interferon. The results show that interferon induces rapid (within 1 h) phosphorylation-dephosphorylation reactions involving ribosome-associated and ribosomal structural proteins of HeLa cells.
用人α干扰素（HuIFN-α）孵育HeLa细胞，导致总核糖体蛋白（核糖体相关蛋白和核糖体结构蛋白）的磷酸化增加，其磷酸化程度高于仅在培养基中孵育的细胞。这些蛋白的最大磷酸化发生在4000单位/毫升的HuIFN-α、细胞与HuIFN-α孵育1 - 2小时以及32P脉冲期为1小时的条件下。将总核糖体蛋白分离为核糖体相关蛋白和80S核糖体结构蛋白，结果显示干扰素诱导的磷酸化增加仅与一种36K核糖体相关多肽有关，且在干扰素处理的细胞中80S核糖体结构蛋白的磷酸化受到抑制。在干扰素处理的细胞中，大、小亚基核糖体结构蛋白磷酸化的抑制水平分别为14 - 19%和76 - 81%。在细胞与干扰素最初孵育2小时后，核糖体结构蛋白磷酸化的抑制持续24小时。干扰素处理抑制了几种与纯化的80S核糖体相关的蛋白的磷酸化。干扰素处理显著降低了HeLa细胞中柯萨奇病毒B3的产量，但在细胞与干扰素孵育10小时期间对蛋白质合成速率几乎没有影响。结果表明，干扰素诱导HeLa细胞中核糖体相关蛋白和核糖体结构蛋白快速（1小时内）发生磷酸化 - 去磷酸化反应。
