巴西利什曼原虫的核糖体DNA：核糖体拷贝数及基因分离

Ribosomal DNA of Leishmania brasiliensis: number of ribosomal copies and gene isolation.

作者信息

Villalba E, Ramírez J L

出版信息

J Protozool. 1982 Aug;29(3):438-41. doi: 10.1111/j.1550-7408.1982.tb05428.x.

DOI:10.1111/j.1550-7408.1982.tb05428.x

Abstract

The DNA content of the hemoflagellate Leishmania brasiliensis, strain Y, has been determined by colorimetric reactions and found to be nearly 0.226 pg/cell. When this DNA is bound to filters and hybridized with labeled rRNA from the same organism, saturation is reached at 0.47% of the DNA, corresponding to an estimated 160 ribosomal gene copies. When the DNA is sheared and centrifuged to equilibrium in CsCl gradients, two major satellites of the main band (rho = 1.712 g/cm3) are observed: a heavy one (1.720 g/cm3), which hybridizes with labeled rRNA, and a light one (1.699 g/cm3) with the electron microscopic characteristics of the kinetoplast DNA network.

摘要

通过比色反应测定了巴西利什曼原虫Y株的血鞭毛虫的DNA含量，发现其约为0.226 pg/细胞。当这种DNA与滤膜结合并与来自同一生物体的标记rRNA杂交时，在DNA的0.47%处达到饱和，相当于估计有160个核糖体基因拷贝。当DNA被剪切并在CsCl梯度中离心至平衡时，观察到主带（ρ = 1.712 g/cm³）的两个主要卫星带：一个重带（1.720 g/cm³），它与标记的rRNA杂交；一个轻带（1.699 g/cm³），具有动质体DNA网络的电子显微镜特征。