Hartmann W, Kluge H
Psychiatr Neurol Med Psychol (Leipz). 1982 Jul;34(7):395-408.
Valuation methods for IgG, IgA, IgM and transferrin in normal and pathologically abnormal CSF samples are described. All proteins were determined by a modified electro-immuno-precipitation technique on cellulose acetate films in CSF which had not been concentrated. The methods for valuating IgG, IgA and transferrin were derived from criteria for the relation between each of the different proteins and the total protein concentration of normal CSF samples. Due to the limits of detection with our equipment, a different evaluation scheme was used for IgM. This was based on the changes in IgM concentration in the CSF which had a total protein content between 500 and 2.500 mg/1. These methods of valuation can be used to interpret changes in the mechanisms of the cerebral barrier function and/or the immunological status of neuropsychiatric diseases.
描述了正常和病理异常脑脊液样本中IgG、IgA、IgM和转铁蛋白的评估方法。所有蛋白质均通过改良的电免疫沉淀技术在未浓缩的脑脊液醋酸纤维素膜上进行测定。评估IgG、IgA和转铁蛋白的方法源自不同蛋白质与正常脑脊液样本总蛋白浓度之间关系的标准。由于我们设备的检测限，对IgM采用了不同的评估方案。这是基于总蛋白含量在500至2500mg/1之间的脑脊液中IgM浓度的变化。这些评估方法可用于解释脑屏障功能机制和/或神经精神疾病免疫状态的变化。
