Hetland O, Warhuus K, Giercksky K E, Aaslid R, Prydz H
Scand J Clin Lab Invest. 1982 May;42(3):239-44.
Tissue thromboplastin is the most potent physiological trigger of blood coagulation and is probably involved in the pathogenesis of several forms of intravascular coagulation. Phospholipase C from Bacillus cereus is an effective inhibitor of thromboplastin. As part of an investigation into the possible use of phospholipase C as a therapeutic or prophylactic agent in thrombosis and other forms of intravascular coagulation, we have previously studied its effect in rats. We now report on the toxicity in rabbits (along with some data on cats and monkeys). Estimated LD50 for rabbits was about 0.45-0.65 mg/kg (as compared to 1.70 mg/kg for rats). No respiratory or circulatory changes were observed, but phospholipase C caused a significant increase of several plasma enzymes in rabbits.
组织凝血活酶是血液凝固最有效的生理触发因素，可能参与多种形式的血管内凝血的发病机制。蜡样芽孢杆菌的磷脂酶C是凝血活酶的有效抑制剂。作为对磷脂酶C作为血栓形成和其他形式血管内凝血的治疗或预防药物的可能用途进行调查的一部分，我们之前已经研究了它在大鼠体内的作用。我们现在报告其对兔子的毒性（以及一些关于猫和猴子的数据）。兔子的估计半数致死量约为0.45 - 0.65毫克/千克（相比之下，大鼠为1.70毫克/千克）。未观察到呼吸或循环变化，但磷脂酶C导致兔子体内几种血浆酶显著增加。
