用于分析电子显微镜的生物冷冻切片制备

Preparation of biological cryosections for analytical electron microscopy.

作者信息

Zierold K

出版信息

Ultramicroscopy. 1982;10(1-2):45-53. doi: 10.1016/0304-3991(82)90186-3.

Abstract

A cooling chain for studies of ultrastructure and elemental composition of cryofixed biological cells and tissues is described. The technique is demonstrated for yeast cells. The preparation steps such as cryofixation, cryosectioning, transfer into the electron microscope as well as imaging and X-ray microanalysis in the STEM are discussed with respect to the present possibilities and problems. Whereas freeze-dried cryosections can be studied routinely, imaging of the ultrastructure and measurement of the elemental distribution in frozen-hydrated sections turn out to be limited.

摘要

本文描述了一种用于研究冷冻固定生物细胞和组织超微结构及元素组成的冷却链。该技术已在酵母细胞上得到验证。文中讨论了诸如冷冻固定、冷冻切片、转移至电子显微镜以及在扫描透射电子显微镜（STEM）中成像和X射线微分析等制备步骤，涉及到当前的可能性和问题。虽然冻干冷冻切片可以常规研究，但冷冻水合切片的超微结构成像和元素分布测量却受到限制。