用于通过扫描透射电子显微镜和X射线微分析对冷冻固定生物标本进行研究的冷却链。

A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis.

作者信息

Zierold K, König R, Olech K H, Schäfer D, Lübbers D W, Müller K H, Winter H

出版信息

Ultramicroscopy. 1981;6(2):181-186. doi: 10.1016/0304-3991(81)90058-9.

Abstract

A cooling chain is described which enables the transfer of frozen hydrated biological specimens (ultrathin cryosections as well as about 1 micrometer thick cultured cells) from a cryoultramicrotome into a scanning transmission electron microscope with a field emission gun. Transfer is done at 118 K, specimen temperature in the microscope is 165 K. Sublimation processes are controlled visually and by mass spectrometry. Electron micrographs and X-ray microanalytical spectra of cryofixed unstained tissue culture cells and rat liver tissue sections are described and discussed. Contamination of the specimen is much reduced by use of the cold stage.

摘要

描述了一种冷却链，它能够将冷冻水合生物标本（超薄冷冻切片以及约1微米厚的培养细胞）从冷冻超薄切片机转移到带有场发射枪的扫描透射电子显微镜中。转移在118K下进行，显微镜中的标本温度为165K。升华过程通过视觉和质谱法进行控制。描述并讨论了冷冻固定未染色的组织培养细胞和大鼠肝脏组织切片的电子显微照片和X射线微分析光谱。使用冷台可大大减少标本的污染。