Lindbom L, Tuma R F, Arfors K E
Acta Physiol Scand. 1982 Jan;114(1):121-7. doi: 10.1111/j.1748-1716.1982.tb06960.x.
The tenuissimus muscle in the rabbit and the cat is a suitable tissue for intravital microscopic investigation of skeletal muscle blood flow. In this study the influence of surgical procedures necessary for direct microscopic observation on the physiological state of the rabbit tenuissimus muscle was assessed by means of blood flow measurements. Mean resting blood flow was 2.8 +/0 0.8 (mean +2- S.D.) ml.min-1.100 g-1 in the left tenuissimus muscle when prepared for microscopic observation as determined by the radioactive microsphere method. This value was not significantly different from that in the intact unexposed muscle in the contralateral leg, 3.3 +/- 1.1. ml.min-1.100 g-1. Exposure of the muscle to atmospheric oxygen tension resulted in a reduction of blood flow to 0.7 +/- 0.4 ml.min-1.100 g -1, suggesting that local metabolic control mechanisms were active. The normal range of vascular control seemed to be maintained, as demonstrated by an increase in blood flow to 64.2 +/- 18.8 ml.min-1.100 g-1 during "maximal" vasodilation induced by topical application of PGE1. The tenuissimus muscle showed a marked sensitivity to mechanical stimulation. Slight stretching of the muscle, similar to what may occur during surgical preparation, resulted in an increase in blood flow to 17.5 +/- 5.7 ml.min-1.100 g-1. Flow values calculated from data obtained by direct microscopic measurements in the tenuissimus muscle agreed well with those obtained by the microsphere method.
兔和猫的薄肌是用于骨骼肌血流活体显微镜检查的合适组织。在本研究中，通过血流测量评估了直接显微镜观察所需的手术操作对兔薄肌生理状态的影响。用放射性微球法测定，准备进行显微镜观察时，左侧薄肌的平均静息血流为2.8±0.8（均值±标准差）ml·min⁻¹·100g⁻¹。该值与对侧腿部完整未暴露肌肉的血流值3.3±1.1ml·min⁻¹·100g⁻¹无显著差异。将肌肉暴露于大气氧张力会导致血流减少至0.7±0.4ml·min⁻¹·100g⁻¹，这表明局部代谢控制机制是活跃的。局部应用前列腺素E1诱导“最大”血管舒张时，血流增加至64.2±18.8ml·min⁻¹·100g⁻¹，这表明血管控制的正常范围似乎得以维持。薄肌对机械刺激表现出明显的敏感性。肌肉的轻微拉伸，类似于手术准备过程中可能发生的情况，会导致血流增加至17.5±5.7ml·min⁻¹·100g⁻¹。通过薄肌直接显微镜测量获得的数据计算出的血流值与微球法获得的值非常吻合。
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