Sultan C, Terraza A, Devillier C, Michel B, Descomps B, Jean R
Br J Dermatol. 1982 Nov;107 Suppl 23:40-6. doi: 10.1111/j.1365-2133.1982.tb01030.x.
Human skin may be considered as a target organ for androgens, since events characteristic of androgen action have been described in this tissue, as well as in cultured human skin fibroblasts. The culture of human skin fibroblasts gives the opportunity to work on living cells, under controlled conditions, in a renewable material from a single skin biopsy. Using this method, we showed the presence od DHT-receptors in the human fetus and we studied the ontogenesis of androgen receptors in relation to sexual differentiation. In the neonatal period, the physiological T rise was not concurrent with a variation in sex skin androgen receptors. The evolution of DHT binding during puberty is now under investigation. These data suggest that the androgen receptor is not modulated by plasma androgens. Androgen receptor determination is of value in defining the biochemical defects involved in partial and complete androgen insensitivity syndrome (twenty-six cases in our study). In idiopathic hirsutism, DHT binding is used to detect an eventual local hypersensitivity. Fibroblast cultures have also been shown to be an excellent model for the screening of compounds which might block the expression of androgen action by competing for the androgen receptors. Cultured skin fibroblasts are a valuable model for the study of androgen and antiandrogen action in human skin.
人类皮肤可被视为雄激素的靶器官，因为在该组织以及培养的人类皮肤成纤维细胞中均已描述了雄激素作用的特征性事件。人类皮肤成纤维细胞的培养为在可控条件下对来自单次皮肤活检的可再生材料中的活细胞进行研究提供了机会。利用这种方法，我们在人类胎儿中发现了双氢睾酮（DHT）受体的存在，并研究了与性别分化相关的雄激素受体的个体发生。在新生儿期，生理性睾酮升高与性皮肤雄激素受体的变化并不同步。青春期期间双氢睾酮结合的演变目前正在研究中。这些数据表明雄激素受体不受血浆雄激素的调节。雄激素受体测定对于确定部分和完全雄激素不敏感综合征（我们研究中的26例病例）所涉及的生化缺陷具有重要价值。在特发性多毛症中，双氢睾酮结合用于检测是否存在局部超敏反应。成纤维细胞培养也已被证明是筛选可能通过竞争雄激素受体来阻断雄激素作用表达的化合物的极佳模型。培养的皮肤成纤维细胞是研究人类皮肤中雄激素和抗雄激素作用的有价值模型。
