通过31P核磁共振进行肝脏代谢研究。

Hepatic metabolism by 31P NMR.

作者信息

Iles R A, Griffiths J R

出版信息

Biosci Rep. 1982 Sep;2(9):735-42. doi: 10.1007/BF01114837.

Abstract

Hepatic metabolism in intact livers has been studied by 31P nuclear magnetic resonance (NMR) spectroscopy. 31P NMR spectroscopy of normal liver detects little ADP and much lower amounts of inorganic phosphate than are found by enzymatic or chemical analysis. Ischaemia of 30 min duration provokes a rapid fall in ATP to undetectable levels; reflow restores the ATP concentration to 70% of its former level. Intracellular pH changes are qualitatively similar. Fructose and glycerol both cause rapid falls in ATP and inorganic phosphate; these original concentrations are almost restored after 30 min in the case of fructose but not with glycerol where they remain depressed.

摘要

完整肝脏中的肝代谢已通过31P核磁共振（NMR）光谱进行了研究。正常肝脏的31P NMR光谱检测到的ADP很少，无机磷酸盐的含量比酶法或化学分析法检测到的要低得多。持续30分钟的缺血会导致ATP迅速下降至无法检测的水平；再灌注可将ATP浓度恢复到先前水平的70%。细胞内pH值的变化在性质上是相似的。果糖和甘油都会导致ATP和无机磷酸盐迅速下降；果糖在30分钟后这些原始浓度几乎恢复，但甘油则不然，其浓度仍处于较低水平。