Raskova J, Morrison A B
Am J Pathol. 1976 Jul;84(1):1-10.
The graft-versus-host (GVH) reactivity of uremic and control spleen cells was studied by popliteal lymph node assay in the rat. The reaction evoked by cells from animals with severe uremia was conspicuously weaker than that evoked by control cells. The magnitude of the GVH reaction induced by control cells was directly proportional to dose, while with the uremic cells the same increases in dose led only to insignificant increases in the strength of the GVH reaction. When mixtures of syngeneic control and uremic cells were used, the GVH reactivity of the control cells was suppressed. The activity of uremic spleen cells can be enhanced (restored) by removal of the sub-population of cells adherent to glass wool. The GVH reaction induced by uremic cells so treated became directly proportional to dose. The removal of the adherent cell population from the uremic spleen cell suspension also led to the disappearance of the suppressor effect in mixtures of control and uremic cells. These results indicate that a decrease in GVH activity of rat uremic spleen cells is due to an increase in suppressor cell activity in the uremic spleen cell population.
采用大鼠腘窝淋巴结试验研究了尿毒症大鼠和对照大鼠脾细胞的移植物抗宿主（GVH）反应性。重度尿毒症动物的细胞所引发的反应明显弱于对照细胞所引发的反应。对照细胞诱导的GVH反应强度与剂量成正比，而尿毒症细胞剂量增加相同幅度时，GVH反应强度仅出现微不足道的增加。当使用同基因对照细胞和尿毒症细胞的混合物时，对照细胞的GVH反应性受到抑制。通过去除附着于玻璃棉的细胞亚群，可增强（恢复）尿毒症脾细胞的活性。如此处理后的尿毒症细胞诱导的GVH反应与剂量成正比。从尿毒症脾细胞悬液中去除贴壁细胞群，也导致对照细胞与尿毒症细胞混合物中的抑制效应消失。这些结果表明，大鼠尿毒症脾细胞GVH活性降低是由于尿毒症脾细胞群中抑制细胞活性增加所致。
