Clerico A, Pilo A, Del Chicca M G, Riente L, Bombardieri S
Chronobiologia. 1982 Jan-Mar;9(1):41-5.
In order to evidentiate whether a circadian rhythm of the myoglobin serum concentration is present in humans, we measured serially the myoglobin levels in blood samples of 21 healthy subjects (10 females and 11 males), at bed rest, by means of a sensitive and accurate radioimmunological method. The data obtained were then evaluated according to the single and mean cosinor analysis, to assess the presence of a significant circadian rhythm. Eighteen of the 21 subjects showed a significant circadian rhythm. The calculated equation for the mean cosinor was: Y = 19.12 + 4.09 cos (15t-128.24) and the F-value for the zero amplitude test was 19.9 (p less than 0.001). Our data demonstrate that circadian variations of myoglobin serum concentration are likely to occur in humans. These findings strongly emphasize the need to take the time of sample collection into account when employing serum myoglobin as a marker for muscle damage.
为了证实人体血清肌红蛋白浓度是否存在昼夜节律，我们采用灵敏且准确的放射免疫法，对21名健康受试者（10名女性和11名男性）卧床休息时的血样中的肌红蛋白水平进行了连续测量。然后根据单变量和平均余弦分析对所得数据进行评估，以评估是否存在显著的昼夜节律。21名受试者中有18名表现出显著的昼夜节律。平均余弦分析计算出的方程为：Y = 19.12 + 4.09 cos (15t - 128.24)，零振幅检验的F值为19.9（p < 0.001）。我们的数据表明，人体血清肌红蛋白浓度可能会出现昼夜变化。这些发现强烈强调，在将血清肌红蛋白用作肌肉损伤标志物时，需要考虑样本采集时间。
