Grandchamp B, Nordmann Y
Enzyme. 1982;28(2-3):196-205. doi: 10.1159/000459102.
An assay for coproporphyrinogen oxidase activity is described which uses a [14C]-coproporphyrinogen substrate with product isolation by methylation, extraction, and thin layer chromatography. This method affords high sensitivity, since a high specific activity of the substrate and good reproducibility due to the incorporation of an internal standard can be obtained. The activity in rat liver and human lymphocytes was found to be 140 nmol protoporphyrin/h/g of liver and 483 pmol protoporphyrin/h/mg of lymphocyte protein, respectively.
本文描述了一种粪卟啉原氧化酶活性测定方法，该方法使用[14C] - 粪卟啉原作为底物，通过甲基化、萃取和薄层色谱法分离产物。由于底物具有高比活性，并通过加入内标实现了良好的重现性，因此该方法具有高灵敏度。结果发现，大鼠肝脏和人淋巴细胞中的活性分别为140 nmol原卟啉/小时/克肝脏和483 pmol原卟啉/小时/毫克淋巴细胞蛋白。
