Edwards P A, Smith C M, Neville A M, O'Hare M J
Eur J Immunol. 1982 Aug;12(8):641-8. doi: 10.1002/eji.1830120804.
A rapidly-growing, HAT-sensitive cell line LICR-LON-HMy2 has been derived from the ARH-77 human plasma cell leukemia-derived line. It lacks the enzyme hypoxanthine phosphoribosyltransferase (EC 2.4.2.8). Hybrids have been reproducibly made for more than a year and in independent laboratories with lymphocytes from tonsils, lymph nodes and peripheral blood and tonsil lymphocytes cultured with antigens. The parent line and hybrids are very robust in culture and double in 20-30 h. Hybrids clone easily and have stable karyotypes, most with modal numbers in the sixties. Stable Ig secretion patterns have been observed over 20-30 passages, and after cloning. It was estimated that about half the hybrids produce new immunoglobulin chains in addition to the parent cell line's IgG1 (kappa light chain). High, but not limiting, density hybridoma cultures (approximately 5 x 10(5) cells/ml) typically produce 0.25-2 micrograms/ml immunoglobulin per day, but some hybrids produce more. A high-secreting variant of LICR-LON-HMy2 has been derived. The LICR-LON-HMy2 line is human and is distinct from the HAT-sensitive human B cell-lines SKO-007 and GM 1500-6TG Al 2. It is available for distribution.
一种快速生长、对次黄嘌呤氨基蝶呤（HAT）敏感的细胞系LICR-LON-HMy2已从ARH-77人浆细胞白血病衍生系中获得。它缺乏次黄嘌呤磷酸核糖转移酶（EC 2.4.2.8）。在一年多的时间里，在独立实验室中，已成功地用来自扁桃体、淋巴结和外周血的淋巴细胞以及用抗原培养的扁桃体淋巴细胞制备了杂交细胞。亲代细胞系和杂交细胞在培养中非常健壮，20 - 30小时内数量翻倍。杂交细胞易于克隆，具有稳定的核型，大多数核型数在60左右。在传代20 - 30次以及克隆后，观察到了稳定的免疫球蛋白分泌模式。据估计，大约一半的杂交细胞除了产生亲代细胞系的IgG1（κ轻链）外，还产生新的免疫球蛋白链。高密度杂交瘤培养物（约5×10⁵个细胞/毫升）通常每天产生0.25 - 2微克/毫升的免疫球蛋白，但有些杂交细胞产生的更多。已获得LICR-LON-HMy2的高分泌变体。LICR-LON-HMy2细胞系是人类细胞系，与对HAT敏感的人类B细胞系SKO-007和GM 1500 - 6TG Al 2不同。它可供分发。
