Miura S, Asakura H, Miyairi M, Morishita T, Nagata H, Tsuchiya M
Digestion. 1982;23(4):224-31. doi: 10.1159/000198754.
The purpose of this study is to examine whether intestinal alkaline phosphatase (IAP) plays a role in transporting lipoproteins to lymphatics during fat absorption. In control rats, the increased activity of IAP in intestinal lymph was observed parallel with the amount of absorbed linoleic acid. In colchicine-treated rats, lymphatic absorption of intraduodenally administered linoleic acid was decreased and administered lipid was transported slowly to lymphatics, mostly in the form of free fatty acid. However, the equal or slightly larger amount of output of IAP to lymphatics was observed in spite of the decrease in lipid absorption in colchicine-treated rats. Histochemically, prolonged activity of IAP in stroma and submucosal lymphatics was found in colchicine-treated rats fed with fat. These results suggest that microtubules are not involved in IAP transport into lymphatics, and these data do not necessarily rule out that IAP plays a role in lipoprotein transport.
本研究的目的是检验肠碱性磷酸酶（IAP）在脂肪吸收过程中是否在将脂蛋白转运至淋巴管方面发挥作用。在对照大鼠中，观察到肠淋巴中IAP活性的增加与亚油酸吸收量平行。在秋水仙碱处理的大鼠中，十二指肠内给予的亚油酸的淋巴吸收减少，给予的脂质缓慢转运至淋巴管，主要以游离脂肪酸的形式。然而，尽管秋水仙碱处理的大鼠脂质吸收减少，但观察到IAP向淋巴管输出的量相等或略多。组织化学上，在喂食脂肪的秋水仙碱处理大鼠的基质和黏膜下淋巴管中发现IAP活性延长。这些结果表明微管不参与IAP转运至淋巴管，并且这些数据不一定排除IAP在脂蛋白转运中发挥作用。
