Bevers E M, Comfurius P, Hemker H C, Zwaal R F
Haemostasis. 1982;12(3):268-74. doi: 10.1159/000214682.
The procoagulant activity of activated platelets in a one-stage prothrombinase assay is reevaluated. It is shown that the apparent procoagulant activity of platelets activated by ADP or collagen can be explained by minor cell lysis accompanying platelet activation. The reduction in clotting time observed with thrombin activated platelets can be explained by a combined effect of minor cell lysis and release and activation of factor V from the platelets. Platelets stimulated by ionophore A23187 or by the combined action of collagen plus thrombin show a much shorter clotting time than can be accounted for by minor platelet lysis or release and activation of factor V from the platelets. The results with this clotting assay essentially confirm previous observations [Bevers et al.: Eur. J. Biochem. 122:429-436, 1982] using a spectrophotometric method with highly purified coagulation factors and a chromogenic substrate to measure the rate of thrombin formation with activated platelets.
在一期凝血酶原酶试验中，对活化血小板的促凝活性进行了重新评估。结果表明，由ADP或胶原激活的血小板的表观促凝活性可以用伴随血小板激活的轻微细胞溶解来解释。用凝血酶激活的血小板观察到的凝血时间缩短可以用轻微细胞溶解以及血小板中因子V的释放和激活的联合作用来解释。由离子载体A23187刺激或由胶原加凝血酶的联合作用刺激的血小板显示出比轻微血小板溶解或血小板中因子V的释放和激活所能解释的凝血时间短得多。这种凝血试验的结果基本上证实了以前的观察结果[贝弗斯等人：《欧洲生物化学杂志》122:429 - 436，1982]，他们使用分光光度法和高度纯化的凝血因子及显色底物来测量活化血小板形成凝血酶的速率。
