Effect of aeration and mixing in various types of cultivation devices on the cell cycle of budding yeasts.

When investigating the effect of aeration capacity KLa of a cultivation device on the cell cycle of daughter cells of Candida utilis it was found that the length of a phase (S + G2) of the cell cycle is influenced by the rate of oxygen transfer. An increase of KLa of a cultivation device achieved by increasing the specific output of mechanical energy for air dispersion and mixing may lead to cell damage and to changes in the cell cycle. The effect of high intensity of aeration and mixing is thus invalidated.