Mitchell J P, van der Ploeg M
Histochemistry. 1982;75(3):327-40. doi: 10.1007/BF00496736.
Nuclear DNA, nuclear protein and nuclear size have been measured in cells of the cortex, pith and vascular tissue from three successive internodes in the stem of Pisum sativum. New techniques of computer-linked cytophotometry were used to measure these parameters simultaneously in both section and squash preparations. In cortical cells no endoreduplicated nuclei were seen in the internodes measured. In cortical cells from the oldest internode measured, a population of large nuclei with the 2C DNA amount was observed which was not present in the younger internodes. In the oldest pith nuclei measured a few 8C nuclei were present, but maturing pith was most characterized by increasing nuclear size and the population of nuclei accumulating with the 4C DNA amount. Polyploid nuclei were present in all of the vascular tissue measured, including the youngest internode. Maturing vascular tissue was also characterized by increasing nuclear size. Nuclear protein measurements demonstrated a close link between nuclear protein and nuclear size and suggest that increased nuclear size, with constant DNA content, may be due to increased nuclear protein. This raises the question of the nature and function of this nuclear protein, perhaps more characteristic of differentiating cells than dividing cells.
已对豌豆茎中三个连续节间的皮层、髓和维管组织细胞中的核DNA、核蛋白和核大小进行了测量。利用计算机连接细胞光度测量新技术，在切片和压片标本中同时测量这些参数。在所测量的节间皮层细胞中未观察到核内复制核。在测量的最老节间的皮层细胞中，观察到一群具有2C DNA含量的大核，而在较年轻节间中不存在。在所测量的最老髓核中有一些8C核，但成熟髓的最显著特征是核大小增加以及积累4C DNA含量的核群体。在所有测量的维管组织中都存在多倍体核，包括最年轻的节间。成熟维管组织的特征也是核大小增加。核蛋白测量表明核蛋白与核大小之间存在密切联系，并表明在DNA含量恒定的情况下核大小增加可能是由于核蛋白增加。这就提出了这种核蛋白的性质和功能问题，它可能在分化细胞中比在分裂细胞中更具特征。
