Sótonyi P, Kerenyi N A, Somogyi E
Histochemistry. 1982;75(3):425-36. doi: 10.1007/BF00496744.
The ortho-cresolphtalein complex was successfully adapted for the electron microscopic cytochemical demonstration of calcium. The reaction product is of granular nature with sufficient electron density for finer localization. Intense precipitation was found on the sarcolemma and transverse tubules and in the sarcoplasmic reticulum. Myofilaments, mitochondria and capillary endothelial cells also showed a positive reaction. The electron microprobe analysis of the precipitate proved the presence of calcium. Disturbing effects of magnesium ions were prevented by the incorporation of 8-hydroxyquinoline in the incubation medium.
邻甲酚酞络合物成功地用于钙的电子显微镜细胞化学显示。反应产物呈颗粒状，具有足够的电子密度以便进行更精细的定位。在肌膜、横小管和肌浆网中发现有强烈沉淀。肌丝、线粒体和毛细血管内皮细胞也呈阳性反应。沉淀物的电子微探针分析证实了钙的存在。通过在孵育介质中加入8-羟基喹啉可防止镁离子的干扰作用。
