胆碱调节离体仓鼠心脏中磷脂酰乙醇胺的生物合成。

Choline regulates phosphatidylethanolamine biosynthesis in isolated hamster heart.

作者信息

Zelinski T A, Choy P C

出版信息

J Biol Chem. 1982 Nov 25;257(22):13201-4.

Abstract

The effect of choline on phosphatidylethanolamine biosynthesis in the isolated hamster heart was investigated. Hamster hearts were perfused with [2-14C]ethanolamine in the presence of 0-0.5 mM choline. Incorporation of label into phosphatidylethanolamine was decreased 28% at 0.2-0.5 mM choline. Analysis of the ethanolamine-containing metabolites of the CDP-ethanolamine pathway revealed that choline (0.2-0.5 mM) in the perfusate had no effect on the labeling of ethanolamine. However, a 28-30% reduction in the labeling of phosphoethanolamine and CDP-ethanolamine was observed. Concurrently, there was a 2-fold increase in the intracellular choline level. Although the formation of CDP-ethanolamine is regarded as the rate-limiting step for phosphatidylethanolamine biosynthesis, choline was found to inhibit ethanolamine kinase, but did not have any effect on phosphoethanolamine cytidylyltransferase. Hence, choline may provide an additional mechanism for the regulation of phosphatidylethanolamine biosynthesis.

摘要

研究了胆碱对离体仓鼠心脏中磷脂酰乙醇胺生物合成的影响。在存在0 - 0.5 mM胆碱的情况下，用[2-¹⁴C]乙醇胺灌注仓鼠心脏。在0.2 - 0.5 mM胆碱时，标记物掺入磷脂酰乙醇胺的量减少了28%。对CDP - 乙醇胺途径中含乙醇胺代谢物的分析表明，灌注液中的胆碱（0.2 - 0.5 mM）对乙醇胺的标记没有影响。然而，观察到磷酸乙醇胺和CDP - 乙醇胺的标记减少了28 - 30%。同时，细胞内胆碱水平增加了2倍。尽管CDP - 乙醇胺的形成被认为是磷脂酰乙醇胺生物合成的限速步骤，但发现胆碱可抑制乙醇胺激酶，而对磷酸乙醇胺胞苷转移酶没有任何影响。因此，胆碱可能为磷脂酰乙醇胺生物合成的调节提供一种额外机制。