Williams R, Gaber B, Gunning J
J Biol Chem. 1982 Nov 25;257(22):13321-3.
The secondary structure of crystalline nerve growth factor (NGF) has been measured with 90% confidence to contain between 0 and 11% helix and between 53 and 67% antiparallel beta-strand using the intensity distribution of the amide I region of the Raman spectrum. A comparison of spectra collected from crystals and solutions confirms that the secondary structure of NGF is the same in these two states. The helical content of NGF as determined by Raman differs substantially from predictions based on sequence. The amide I band of NGF is substantially different from that of insulin, indicating that no structural hemology is likely to exist between these two proteins.
利用拉曼光谱酰胺I区域的强度分布，已以90%的置信度测得结晶神经生长因子（NGF）的二级结构含有0%至11%的螺旋结构以及53%至67%的反平行β-链。对从晶体和溶液中收集的光谱进行比较，证实了NGF在这两种状态下的二级结构相同。通过拉曼光谱测定的NGF螺旋含量与基于序列的预测结果有很大差异。NGF的酰胺I带与胰岛素的酰胺I带显著不同，表明这两种蛋白质之间可能不存在结构同源性。
