Hayman E G, Engvall E, A'Hearn E, Barnes D, Pierschbacher M, Ruoslahti E
J Cell Biol. 1982 Oct;95(1):20-3. doi: 10.1083/jcb.95.1.20.
A novel procedure that detects adhesive proteins in complex mixtures was used to characterize such proteins in plasma. The proteins are separated by SDS PAGE and transferred to nitrocellulose filters. Cells incubated on these filters attach to those proteins that have adhesive properties. When applied to human plasma proteins this procedure reveals, in addition to fibronectin, a cell-attachment protein with a polypeptide molecular weight of 70,000. Using a monoclonal antibody that inhibits attachment of cells to fibronectin, we show that this polypeptide is not a fragment of fibronectin and we present evidence that it is a component of the serum spreading factor. Therefore, as defined by our assay, this protein and fibronectin are the major attachment proteins for fibroblastic cells in plasma or serum.
一种用于检测复杂混合物中黏附蛋白的新方法被用于表征血浆中的此类蛋白。这些蛋白通过SDS-PAGE分离并转移至硝酸纤维素滤膜上。在这些滤膜上孵育的细胞会附着于具有黏附特性的蛋白上。当将该方法应用于人类血浆蛋白时，除纤连蛋白外，还揭示出一种多肽分子量为70,000的细胞黏附蛋白。使用一种抑制细胞与纤连蛋白附着的单克隆抗体，我们证明该多肽不是纤连蛋白的片段，并提供证据表明它是血清扩散因子的一个组分。因此，根据我们的测定方法，该蛋白和纤连蛋白是血浆或血清中纤维母细胞的主要黏附蛋白。
