Krahn M M, Malins D C
J Chromatogr. 1982 Oct 15;248(1):99-107. doi: 10.1016/s0021-9673(00)83741-3.
Metabolites of several two- and three-ring aromatic hydrocarbons (AHs) have been found in livers of English sole exposed to No. 2 fuel oil. Four metabolites of the C2H5-naphthalenes, six of the C3H7-naphthalenes and one each of fluorene, phenanthrene and anthracene have been partially characterized and their concentrations, which ranged from 50 to 1100 ng/g, were determined. Metabolites were separated from the liver matrix using an automated extractor/concentrator. The resulting extract was then purified by high-performance liquid chromatography, and the metabolites were characterized and quantitated by gas chromatography-mass spectrometry.
在暴露于2号燃料油的星点木叶鲽肝脏中发现了几种二环和三环芳烃(AHs)的代谢物。已对乙基萘的四种代谢物、丙基萘的六种代谢物以及芴、菲和蒽各一种代谢物进行了部分表征,并测定了它们的浓度,浓度范围为50至1100纳克/克。使用自动萃取器/浓缩器将代谢物与肝脏基质分离。然后通过高效液相色谱法对所得提取物进行纯化,并通过气相色谱-质谱法对代谢物进行表征和定量。