Chilosi M, Pizzolo G, Janossy G, Bofill M, Fiore-Donati L
J Clin Pathol. 1982 Nov;35(11):1220-6. doi: 10.1136/jcp.35.11.1220.
A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether enzyme-histochemical techniques are also feasible on such material. A selected group of enzymes, some of which are inhibited or destroyed in paraffin- or plastic-embedded samples, have been demonstrated. The morphological details obtained were satisfactory in the preparations. The enzymes were dipeptidyl(amino)peptidase IV (for T lymphocytes); tartrate-resistant acid phosphatase (for hairy cell leukaemia); acid phosphatase and non-specific esterase (for macrophages and monocytes); ATPase and 5'nucleotidase (for B lymphocytes); and peroxidase or chloroacetate esterase (for granulocytic cells). In these preparations strong enzyme activities were shown. In adjacent sections the immunological analysis of membrane markers could also be performed contributing to a comprehensive study of the normal and malignant bone marrow cells.
最近开发的一种程序已被证明适用于详细的免疫组织学分析，现用于制备骨髓冰冻切片，以研究酶组织化学技术在此类材料上是否也可行。已证实了一组选定的酶，其中一些酶在石蜡或塑料包埋样本中会被抑制或破坏。所获得的形态学细节在制备物中令人满意。这些酶包括二肽基（氨基）肽酶IV（用于T淋巴细胞）；抗酒石酸酸性磷酸酶（用于毛细胞白血病）；酸性磷酸酶和非特异性酯酶（用于巨噬细胞和单核细胞）；ATP酶和5'核苷酸酶（用于B淋巴细胞）；以及过氧化物酶或氯乙酸酯酶（用于粒细胞）。在这些制备物中显示出较强的酶活性。在相邻切片中，还可以进行膜标志物的免疫分析，有助于对正常和恶性骨髓细胞进行全面研究。
