Pizzolo G, Chilosi M, Fiore-Donati L, Janossy G
Haematologia (Budap). 1984;17(2):247-57.
Cryostat sections from frozen samples of human bone marrow trephine biopsies were used for immunohistochemical analysis. Immunoperoxidase and immunofluorescence techniques as well as enzyme histochemistry were applied to these sections in order to characterize bone marrow infiltrates in lymphoproliferative diseases. Various conventional antisera and monoclonal antibodies and several enzymes were able to identify different normal and malignant cell populations. This technology provides a new tool to be used in combination with classical morphology for studying bone marrow involvement during lymphoid malignancies.
取自人骨髓环钻活检冷冻样本的低温恒温器切片用于免疫组织化学分析。免疫过氧化物酶和免疫荧光技术以及酶组织化学被应用于这些切片，以表征淋巴增殖性疾病中的骨髓浸润情况。各种传统抗血清、单克隆抗体和几种酶能够识别不同的正常和恶性细胞群体。这项技术提供了一种新工具，可与经典形态学结合使用，用于研究淋巴恶性肿瘤期间的骨髓受累情况。
