Hodgkinson V H, Patton C L
J Infect Dis. 1982 Dec;146(6):758-62. doi: 10.1093/infdis/146.6.758.
An extensive fibrillar surface was identified in situ by transmission electron microscopy on the amastigote stage of the macrophage intracellular parasite, Leishmania enriettii. Attachment to the amastigote surface of 20-160-nm fibrils was clearly evident on organisms that had been isolated from guinea pigs. Surface fibrils were not detected after in vitro transformation of the amastigote to the promastigote. Incubation of amastigotes in heat-inactivated guinea pig serum containing a high titer of antibodies to L. enriettii followed by exposure to ferritin-conjugated antibody to guinea pig IgG resulted in labeling of surface fibrils, a result indicating that these fibrils are of parasite origin. Shedding of labeled fibrils was also evident.
通过透射电子显微镜在巨噬细胞内寄生虫利什曼原虫（Leishmania enriettii）的无鞭毛体阶段原位鉴定出广泛的纤维状表面。在从豚鼠分离出的生物体上，20 - 160纳米纤维附着在无鞭毛体表面的情况清晰可见。无鞭毛体在体外转化为前鞭毛体后未检测到表面纤维。将无鞭毛体在含有高滴度抗利什曼原虫抗体的热灭活豚鼠血清中孵育，然后暴露于铁蛋白偶联的抗豚鼠IgG抗体，导致表面纤维被标记，这一结果表明这些纤维起源于寄生虫。标记纤维的脱落也很明显。
