Chourrout D
J Exp Zool. 1982 Oct 10;223(2):175-81. doi: 10.1002/jez.1402230209.
Gynogenetic rainbow trout lines can be produced easily with the simple UV sperm irradiation technique detailed in this study. The dose effect on embryonic survival rate is called a pseudo "Hertwig effect" because of major differences with results of gamma irradiation: Some mixing of various karyotypes (from diploid to haploid number) were obtained with mean doses, probably because of the weak penetrating power of the ultraviolet rays. Irradiations lasting 4 minutes of more are required to produce homogeneous haploid progeny; heat shock applied to such fertilized eggs results in diploid fry of the maternal phenotype.
利用本研究中详述的简单紫外线精子照射技术，可轻松培育雌核发育虹鳟品系。由于与伽马射线照射结果存在重大差异，对胚胎存活率的剂量效应被称为伪“赫特维希效应”：使用平均剂量可获得各种核型（从二倍体到单倍体数）的某种混合，这可能是因为紫外线的穿透能力较弱。需要照射4分钟或更长时间才能产生同质单倍体后代；对这种受精卵施加热休克会产生母本表型的二倍体鱼苗。
