Higgins P J
Pharmacology. 1982;25(3):170-6. doi: 10.1159/000137739.
The effects of the differentiation-inducing agent dimethylsulfoxide (DMSO) on the growth and protein composition of murine hepatic tumor cell (BW77-1) cultures were investigated using various concentrations of the polar solvent in the growth medium. A 4-day exposure to DMSO in concentrations of 0.5, 1 and 3% reduced final hepatocyte population density to 63, 43 and 15% of control values, respectively, while the amount of transferrin in the cellular and extracellular compartments increased in a dose-dependent fashion. The biochemical basis for elevated transferrin extractable per 10(6) BW77-1 cells in DMSO-stimulated cultures was due to increases in both the transferrin contribution to total cellular protein and the mean protein content per cell. This stimulation in cellular protein levels was reflected in the incorporation of [3H]-amino acids into cell-associated 10% trichloroacetic acid-insoluble material. Cultures of transformed liver epithelial cells thus appear to respond to in vitro DMSO exposure by increasing their accumulation of a late-stage hepatocyte gene product.
使用生长培养基中不同浓度的极性溶剂二甲基亚砜（DMSO），研究了分化诱导剂DMSO对小鼠肝癌细胞（BW77-1）培养物生长和蛋白质组成的影响。在0.5%、1%和3%浓度的DMSO中暴露4天，最终肝细胞群体密度分别降至对照值的63%、43%和15%，而细胞内和细胞外区室中转铁蛋白的量呈剂量依赖性增加。在DMSO刺激的培养物中，每10(6)个BW77-1细胞可提取的转铁蛋白升高的生化基础是转铁蛋白在总细胞蛋白中的占比增加以及每个细胞的平均蛋白含量增加。细胞蛋白水平的这种刺激反映在[3H]-氨基酸掺入与细胞相关的10%三氯乙酸不溶性物质中。因此，转化的肝上皮细胞培养物似乎通过增加其晚期肝细胞基因产物的积累来响应体外DMSO暴露。
