Lane N J, Swales L S, David J A, Sattelle D B
Tissue Cell. 1982;14(3):489-500. doi: 10.1016/0040-8166(82)90042-8.
The nicotinic acetylcholine receptor probe alpha-bungarotoxin (1.0 x 10(-7) M) blocks the depolarising response to ionophoretic application of acetylcholine onto the cell body membrane of the fast coxal depressor motoneurone (Df) of desheathed cockroach (Periplaneta americana) metathoracic ganglia, but at the same concentration is completely ineffective in blocking the depolarising action af acetylcholine on dorsal unpaired median (DUM) neurones in the same ganglion. The possibility that this is due to differences in accessibility of the toxin to the neurones has been tested by a combination of ionophoretic injection of horseradish peroxidase into single neurones with a study of the distribution of the exogenous tracer lanthanum, which is of similar effective size to alpha-bungarotoxin. The peripherally located cell body membranes and the fine axonal processes of Df and DUM neurones of desheathed metathoracic ganglia are equally accessible to lanthanum. Differential accessibility to the two cell types does not account therefore for the differences in sensitivity to alpha-bungarotoxin.
烟碱型乙酰胆碱受体探针α-银环蛇毒素（1.0×10⁻⁷ M）可阻断对去鞘蟑螂（美洲大蠊）后胸神经节中快速髋屈肌运动神经元（Df）胞体膜进行离子电泳施加乙酰胆碱时所产生的去极化反应，但在相同浓度下，对阻断同一神经节中乙酰胆碱对背侧无对正中（DUM）神经元的去极化作用却完全无效。通过将辣根过氧化物酶离子电泳注入单个神经元，并结合对与α-银环蛇毒素有效大小相似的外源性示踪剂镧的分布研究，来检验这是否是由于毒素对神经元的可及性差异所致。去鞘后胸神经节的Df和DUM神经元位于外周的胞体膜以及细轴突过程对镧的可及性是相同的。因此，两种细胞类型的可及性差异并不能解释对α-银环蛇毒素敏感性的差异。
